How to extract ev's from blood plasma

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The process for extracting EV's from blood plasma is not straightforward. However, the core principle relies on size exclusion chromatography: blood plasma is centrifuged twice to remove platelets, and stored at -80. When needed, it is divided through a SEC column (technical term: eluted, resulting samples are eluates).1.

  1. Journal of Thrombosis and Haemostasis, 16, 1423-1436 (Libregts, ..., E.N.M. Nolte-'T Hoen, ...) ↩

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Jobs theory and customer journeys
Compress a file or directory with tar
Learning in Public
scipreneurs #productmarketfit #customeroriented
Essays/linkedin/23-12-21 things that can scale
Essays/linkedin/23-12-01 uncertainty and risk
Education allows upwards social mobility
Waterfall development
Difference between detection and tracking of nanoparticles
Literature/202311111406 policies for a net zero future
Webmentions
How Does Online User Tracking Works
Not all developers are computer savvy
Literature/@scotte
Replacing analysis with statistics
Literature/202103131212 aav2 capsids suffer from aggregation making them unstable and harder to use for medical applications
Quantifying the concentration of evs in urine or plasma through confocal microscopy
Difference between risk and uncertainty
Neanderthal dna deserts
Subscription Economy
Cytometry
Implementing webmentions
Create the need for a product
Lifecycle of a sicentific idea
Literature/202311111404 staying warm and cool
202404281926
Successful open projects are those that last longer
Essays/how to incentivize a scipreneurial path
The power of flow cytometry
In spaced repetition not all tasks have the same weight
Concept Summary
Scipreneurs/7-day-intro/6 - can you stay motivated
Innovation leads to growth
Circulating tumor cells
Washburn's Equation
Choosing pycharm over vscode
Notes on "How to take Smart Notes" by Sönke Ahrens
Effective feedback gathering
Resegmented existing market positioning
Literature/202303251644 ideas-first product innovation is flawed
Chaos - james gleick
Communication channels for citizen science projects
Essays/linkedin/24-05-06 six sigma
Companies working on carbon capture
Patents can be awarded to ideas without empiric verification
Public engagement with research is challenging
Funding Your Venture: Grants, Investors, and Beyond
Evs that inhibit viral infection
Product market fit with biased data
Scipreneurs/7-day-intro/7 - moving forward
Treacy-wiersema frame
Scipreneurs/book/00 index
Bullet journaling
Five stages of colloidal particle tracking
Gender neutral infrastructure favors men
Number of contact points in marketing
Fundamental Attribution Error
Agile development
Founders have a fundamental role in customer discovery
Effective interventions to make more published research true
Work policies designed for the average
Tres Truenos, de Marina Closs
Gender Diversity in Academia
Inquiry-based science education
Is openspim a good example of open hardware
Nanosight
Market research is a commodity
Innovation is not the only driver for success
Knowledge is a positive externality
Is Github contradictory of open source values?
Compressive raman
Los tres incendios de la bilbioteca de alejandría
Customer creation
Los romanos adoptaron la cultura griega
The leader in you - dale carnegie
Como Agua para Chocolate - Laura Esquivel
Mbfcm
Podcast I listened in Week 32
Challenges with studying corrosion
Cd9
Kanban development
Teachers lack tools to design a science curriculum
Essays/linkedin/24-05-08 patents
What are patents for
Klimaat helpdesk
El secreto de Antoni
Attribution-ShareAlike 4.0 International (CC BY-SA 4.0)
Competitor analysis
Work in progress
Common problems with open source projects
Kano model
Effectual Reasoning
Sampling error when measuring the average diffusion coefficient
Science in the classroom is separated from the real world outside
The biases each one of us carries
Technical Inequality
Terrarium closed for 50 years is life's microcosm
Literature/202311280955 socialize risks and privatize rewards
shinrin-yoku
Literature/202311281141 companies will invest more on applied research
Continuity is required by persistent users
Literature/202311280944 the fake speech of free market in innovation
Literature/202306121143 open hardware is not all about money
Literature/202301141616 membrane-binding peptides to detect small evs
The value of code
Patents can limit the positive externalities of research products
Literature/202311101914 the problem with electricy
Democracy and algorithms
202304271736
Literature/202310101212 stereo darkfield interferometry
Personal data collection, what is it and where it starts
Literature/202301241150 different modes of operation of microfluidic free-flow electrophoresis
Role of introgression to define the percentage of modern and neanderthal genome
Cumulative culture
Code Before you Think
Literature/@beal1956
Determining drug loading and release kinetics in nanoparticles
Literature/202311111345 net zero agriculture
Depletion of the low-hanging fruit in science
Literature/202301141333 comparing simoa and sp-iris
Social networks exploit the reward equation
Literature/@iso 18069 2015
Literature/202212281435 polydisperse samples with inta
Science Education
Do Things That don't Scale
Git is a poorly designed tool
Mentor, Consultant, Coach, Partner: Who Do You Need Right Now?
Literature/202306221512 sers to identify cancer evs
Essays/ splitting shares of a startup should reflect future work
Scientific products
Science only works in the Class Room
Literature/202404021200 free flow electrophoresis for plasma processing
Tech Notes
Literature/202410012120 product vision
Formatting strings in two steps with Python
scipreneurs #scientists-entrepreneurs #market-size
Web Mentions and comments for websites
Starting a company is like jumping off a cliff and assembling a plane on the way down
Citations make science conservative
Minimum viable product
High value ideas are based on research and science
Understanding and Accepting the Risk of Failure
My phd
Model view controller
Literature/202311280851 tracking charges in lithium electrodes
Bias towards men problems means less research focused on women health
Customer journey and touchpoints
Entrepreneurship for a pi is very different than for a phd or postdoc
Aquiles.me newsletter
Scipreneurs/7-day-intro/1 - know yourself
Literature/202311101921 the biggest problem is making things
Measure the exploration factor of research
Increase the number of frames for tracking by confining the movement of a nanpoarticle
Holocene
202409160819
Literature/202310112003 acoustic force spectroscopy for single-molecule applications
Playbook Entrepreneurship for Academics
Resonance calendar/ init .py files are optional
The only way to get the best of an argument is to avoid it
How Technology can Shape Education
It is not clear the amount of farming done by women
The Liberation of RNA (podcast)
Try honestly to see things from the other person's point of view
Scipreneurs/7-day-intro/0 - welcome message
Building and Leveraging Your Network: Engaging with Key Opinion Leaders (KOLs)
How to Remember how to Link notes in a Digital Garden
Literature/202303251644 needs-first product innovation is flawed
Mi Rincón en Internet
Tetraspanins are unevenly distributed across extracellular vesicles
Bikepacking
Literature/202212291648 optical methods for single molecule analysis
Ev targeting
Psa
Having a patent does not entail competing interests
Privacy Policy
Open hardware business model
Build trust in a team
Deep tech
Don't criticize, condemn, or complain
City planning with biased data
Hermeneutic circle
Breaking the Chronological Order
Quantification of protein interactions and solution transport using high-density GMR sensor arrays
Piloting a new venture
Device experiment view
Embracing the Scipreneur Mindset: Transitioning from Scientist to Entrepreneur
Job titles can stunt startup development
Adapting Academic Skills for Business Use
Post hoc ergo propter hoc
Public engagement with research activities
Pricing products
Open science
Identifying and Validating Market Opportunities
Life cycle of the malaria parasite
Modern monetary theory
Eccentric scientist type
Pleistocene
Tracking Scripts
Luhmann method
Nieve de primavera - Mishima
Overkill hypothesis
Brilliance bias
Las máquinas no pueden soñar
Interruption marketing
Let the other person do a great deal of the talking
Microvesicles
Phases of customer validation
Product market fit for scientific instrumentation
Literature/202111292230 diesel is the most efficient energy storage solution for transport
The effect of the track length on the accuracy of the diffusion coefficient in MSD measurements
Motivation factors for scientific entrepreneurship
Literature/202111302113 quantifying sources of error in nanoparticle tracking analysis
Develop a combination of methods to measure particle size distribution of medical nanoparticles
Low abundance evs
Market requests for a technology
Mbti personality indicator
NSM
Marketing blogs are not meant to be a digital garden
What is Blockchain
Determining the best number of fitting points for MSD data
Hypertext today is only a part of what it could have been
Nanoscale deterministic lateral-displacement pillar arrays
I deleted my Facebook account
Requirements of a Lab Journal
mvp #hardware #scipreneurs #techtransfer
Essays/professors can enable scipreneurs
Problems with citation-based metrics
Having sex-disaggregated data is the only way to build consistent tax policies
La Inevitabilidad de Envejecer
Optical chromatography
Make the other person feel important - and do it sincerely
Science education should be method-oriented
Technolgy transfer
Make decisions with incomplete information
Cell-mediated immunity
Process of Automatizing
Writing May be the most Undervalued Skill
Prakash Lab
Literature/@bloom2020
Nasdaq is fundamental for quicker exits
Pareto Principle (Mental Model)
Essays/do things that can scale
GAFA CEO's testify in front of the US Congress
Miss out opportunities because of internal culture
Pleistocene extinctions
Schizogony
Open hardware
§ how i take notes of papers
El cambio incremental nos puede llevar hacia lugares indeseados
Lycurgus cup
§-notes
Dewey decimal classification
Open source checklist before publishing a paper
We are Craving for Data
Reversing Science Education
Simple explanation of an optimization algorithm
Optimal number of fitting points for msd measurements
Why we teach science in school
Illumion
Self-Driving Cars
How to take smart notes - sönke ahrens
Ovsiankina effect
Self-Host My Newsletter
My cv made into a podcast
Objections to purchase
Calibration services for open-hardware tools
Limitations of nta for extracellular vesicles measurements
Essays/linkedin/24-05-22 before accepting
Nanoparticle tracking analysis
Obsidian
Overload of information is pushing us to spaces with restricted access
Teachers bring their own knowledge gaps to the classrooms
Correlation between pedagogical approach and science education outputs
Hiring a copy editor for python for the lab
Customer development
Swot analysis
Four insights of great managers
Revert to the Last Known Working State
Plurality of online spaces
failing #startups #entrepreneurs
Existing market marketing
Cells behave differently in the body than on rigid substrates
Product lifecycle
Linking particle positions to form trajectories
The world in 2050 if we don't stop the heating
The golden circle
Dispertech
The hidden power of individual reviewers
Mitovesicles
Startups
Quantifying performance using multiple measurements
Science education is utilitaristic
Product development
Why footnotes should be side notes on websites
Venture capitalists
Things I've Done
Cartesian dualism
Build - Tony Fadell
Everyone can Build a Twitter Audience
Catalytic experience of invitae
Venture capital is more short-termist than what research needs
Limits of time-based separation methods
Spaced repetition
Cd81
Using scattering instead of fluorescence
The fallacy of the carbon emitters
Ccmv virions
Hipster hacker hustler
Backlinks
The three worlds of the andean cosmos
Primary education to establish the basis of scientific thinking
Business model prototypes
Ciencia ciudadana
The interaction between Stackoverflow and Github
The Economy of Open Source Software
Private money in universities
The Eleventh Commandment - Jeffrey Archer
Ultra centrifugation
Effectual bird in the hand
Exosomes
Lowering the Maintenance burden of open source projects
Patents and spin-offs do not have the same added value
After a Failure, Revert to the Last Known Working State
The 3-month scipreneurial path
Technology push
Catalytic experience of ge healthcare life sciences
What is a Machine Learning Algorithm
Novelty is not Innovation
Video Tutorials
Basic competences that define scientific literacy
Open Source is not what it was
The traditional marketing funnel
Erenna
Cd9 and cd63 are used as biomarkers in evs but they can change from cell line to cell line
Patents
Dunning-kruger effect
The Web is built on standards
Cuantificar lo que no sabemos
The sociology of lockdowns
Things I Want to Do
Ask why
El Problema de los Tres Cuerpos - Liu Cixin
Negative 2000 lines of code
Building reputation
Catalytic experiences - hamid ghanadan
There is a discrepancy in the reported concentration between nta and evquant
Choosing between Zettlr and Obsidian
About this Website
Science education should not distinguish disciplines
Prostate cancer
Brownian Diffusion
Literature/@boccara2016
Nanosight proprietary software update
Hemozoin
Citizen science in schools
Why not all my e-mails are signed
Losing Jobs in the Hands of Artificial Intelligence
Talk in terms of the other person's interests
Subscription-Based Product
The problem with people in positions of power in academia
Teaching curricula in latin america fail to guide teachers
Literature/@rieu2021
Limitations of fluorescence for particle tracking
Resonance Calendar
Making Myself Available for a Talk
Stokes-einstein relationship
Gray hair fallacy
Machine learning
Uncover the desire
Literature/@meyvis2021
Literature Notes
Utrechtinc and its copy-pasting approach
Literature/@ianuzzi2017
Structure of a digital garden
Effect of vibration in diffusion coefficient determination
The scientific risks of professors holding shares in companies
Use of hydrogels for medicine
Medicine excludes women from their studies yielding biased results
The Uberization of the Workforce
Literature/@dent2006
Female hunters in hunter-gatherer societies
Ways of delivering value
Egalitarian societies explain diveristy in hunter-gatherer groups
Reforestation
202405221743
V1 is not even remotely profitable
Bioenergy with Carbon Capture and Storage
Education is content based
Why would you learn how to open source projects?
Tracking polystyrene particles
The problem with the power of web developers
It is challenging to distinguish hiv viruses from evs
Why implementing OKRs
Business models for research based tools
Essays/ skill building
Literature/@kim2015
Xiaomei yan
Literature/open-source miniature spectrophotometer (osms)
Essays/linkedin/23-12-14 think like a designer
Scientific publishing is the largest experiment on backlinking we have access to
Literature/@laganovska2020
Uncertainty in scientific exploration
Analog versus digital detection of molecules
Literature/@scotte2020
Literature/@li2022a
Appeal to the nobler motives
Essays/linkedin/ misconception 7 you need a ceo
Literature/202301192057 free flow electrophoresis
Literature/202311281010 states are investing in lowering risks
Science education could focus on understanding the world around people
Literature/202311281015 innovation led policies
Literature/202011092209 writing projects should flow associatevely and not with a top-down approach
Literature/@elsharkasy2020
Literature/@lam2011
Start with why
Size exclusion chromatography is the second most popular ev enrichment method
Images/pasted
Images/mentor
Value-based selling
Single-molecule detection with a 3d printed microscope

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