There is a discrepancy in the reported concentration between nta and evquant
There is a discrepancy between measurements based on nanoparticle tracking analysis and the EVQuant protocol[@hartjes2020EVQuant; high-throughput quantification and characterization of extracellular vesicle (sub)populations]. (See: quantifying the concentration of EVs in urine or plasma through confocal microscopy)
The NanoSight is not a reliable instrument for small particles, given that they diffuse very quickly (see: Sources of error in measuring diffusion coefficient through nanoparticle tracking analysis). On the other hand, if the samples are immobilized and imaged through a confocal microscope, it is possible to adjust the settings to become sensitive even to single-molecules.
Backlinks
These are the other notes that link to this one.
Nothing links here, how did you reach this page then?
Comment
Share your thoughts on this note. Comments are not public, they are
messages sent directly to my inbox.
Aquiles Carattino
This note you are reading is part
of my digital garden. Follow the
links to learn more, and remember that these notes evolve over time. After all,
this website is not a
blog.