Ev isolation and purification
There are different methods that are commonly used for isolation and purification of EVs ([@ramirez-garrastacho2021]):
- Differential centrifugation
- ultra centrifugation
- Acoustic trapping
- size exclusion chromatography
- Nanoscale deterministic lateral-displacement pillar arrays
However, there tends to be a mismatch between isolation and measurement techniques[@ramirez-garrastacho2021].
The most important aspect to always keep in mind is that in any isolation or purification step there are extracellular vesicles being lost, there is the chance of inducing a bias in the results. For instance, if the method enriches particles bigger than a given diameter, or with a minimal surface charge, or molecular composition.
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