Increase the number of frames for tracking by confining the movement of a nanpoarticle
Instead of performing nanoparticle tracking analysis in free diffusion, one can confine the movement to a smaller geometry such as a 1D channel in order to improve the accuracy of the detection by increasing the total number of acquired frames, and the localization accuracy by lowering the background (@Faez2015Fast, Label-Free Tracking of Single Viruses and Weakly Scattering Nanoparticles in a Nanofluidic Optical Fiber).
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