Limitations of fluorescence for particle tracking

<p>Fluorescent dyes offer a limited photon budget (<a class="litnote tooltip" href="/literature/@fo2020">@fo2020<span class="tooltiptext">Tracking and Analyzing the Brownian Motion of Nano-objects Inside Hollow Core Fibers</span></a>): the fluorescence lifetime limits the maximum rate at which photons can be emitted, and <em>photobleaching</em> limits the total number of photons that can be emitted overall. Therefore, there is a tradeoff between amount of photons per second and overall length of the experiment. In the case of <a class="wikilink" href="/nanoparticle_tracking/">nanoparticle tracking</a>, this affects the accuracy of the localization and the number of frames over which a particle can be tracked through fluorescence (see: <a class="wikilink" href="/limitations_of_nanoparticle_tracking_analysis/">limitations of nanoparticle tracking analysis</a>).</p>