Literature Note

Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations

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Author list
  • Rissin, David M.
  • Kan, Cheuk W.
  • Campbell, Todd G.
  • Howes, Stuart C.
  • Fournier, David R.
  • Song, Linan
  • Piech, Tomasz
  • Patel, Purvish P.
  • Chang, Lei
  • Rivnak, Andrew J.
  • Ferrell, Evan P.
  • Randall, Jeffrey D.
  • Provuncher, Gail K.
  • Walt, David R.
  • Duffy, David C.
Rissin et al. increase the sensitivity of sandwich ELISA by segregating beads bearing a single enzyme-labeled immunoconjugate into femtoliter-volume reaction chambers. As the small volume of each well permits detection of extremely low levels of fluorescence, protein abundance is determined by counting the number of fluorescent wells as a percentage of the number of wells containing beads.

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