Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations
This is a literature note auto-generated from bibliographic records.
DOI:10.1038/nbt.1641
Author list
- Rissin, David M.
- Kan, Cheuk W.
- Campbell, Todd G.
- Howes, Stuart C.
- Fournier, David R.
- Song, Linan
- Piech, Tomasz
- Patel, Purvish P.
- Chang, Lei
- Rivnak, Andrew J.
- Ferrell, Evan P.
- Randall, Jeffrey D.
- Provuncher, Gail K.
- Walt, David R.
- Duffy, David C.
Rissin et al. increase the sensitivity of sandwich ELISA by segregating beads bearing a single enzyme-labeled immunoconjugate into femtoliter-volume reaction chambers. As the small volume of each well permits detection of extremely low levels of fluorescence, protein abundance is determined by counting the number of fluorescent wells as a percentage of the number of wells containing beads.