Measure the protein adsorption to MedNPs
- Source: [ @gioria2018 Are existing standard methods suitable for the evaluation of nanomedicines: some case studies ]
- Tags: #nanomedicine #mednp #medical-nanoparticles
Measuring the size-distribution of nanoparticles (see: 202111301045 particle size distribution of medical nano-particles ) can be important to determine the protein adsorption to MedNPs. When particles are incubated in cell culture media, or serum, protein adsorption can lead to changes in the sample, including aggregation, changes in morphology, or even breakdown.
Drugs such as Doxil (the first approved nanomedicine in the US) are PEGylated to avoid interacting with proteins in blood. By accurately measuring the size of the particles, it could be possible to measure protein adsorption to the surface.
The challenges lie in the impossibility of having access to the ground truth. TEM images do not see the protein corona, and NTA will struggle to recognize any changes since the refractive index of the protein is very similar to that of the medium. AF4-DLS-MALS shows some promising results in identifying changes in particle’s shape and size when exposed to complex media.
Measuring stability is of utmost importance, since drug delivery kinetics are highly dependent on morphology.
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