Quantification of protein interactions and solution transport using high-density GMR sensor arrays

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Schematic of GMR sensor arrays and protein binding

Studying protein dynamics is crucial for many aspects of biology, from DNA hybridization, antigen-antibody binding, and DNA-protein interaction. However, one of the challenges is that the introduction of labels alter the behavior of the studied object (for instance, the diffusion coefficient changes, etc.)

The paper by [ @gaster2011 Quantification of protein interactions and solution transport using high-density GMR sensor arrays ] focuses on using magnetic nanoparticles in combination with giant magnetic resistance sensors. A functionalized surface can trap the magnetic beads and their presence can be detected in parallel, in real-time .

The core message of the work is the development of a model to characterize the protein dynamics between labeled objects and a surface. The model is semi-analytical and seems to describe properly the detected signals.

The biggest advantage over surface plasmon resonance sensors is the large dynamic range (6 log or more compared to 2 log for SPR), and the concentration: $$25\textrm{ng}\,\textrm{ml}^-1$$ versus $$1\textrm{pg}\,\textrm{ml}^-1$$.

This work opens the door to profiling the affinity of specific compounds against an entire proteome.

There is a spinout associated with the project called Flux Biosciences


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