Quantification of protein interactions and solution transport using high-density GMR sensor arrays

<ul> <li> Source: [ <a class="litnote tooltip" href="/literature/@gaster2011"> @gaster2011 <span class="tooltiptext"> Quantification of protein interactions and solution transport using high-density GMR sensor arrays </span> </a> ] </li> <li> Tags: <a href="/tags/giant-magnetic-resistance"> #giant-magnetic-resistance </a> <a href="/tags/biosensor"> #biosensor </a> <a href="/tags/protein-dynamics"> #protein-dynamics </a> </li> </ul> <p> <img alt="Schematic of GMR sensor arrays and protein binding" src="/images/GMR_sensor_arrays.png"/> </p> <p> Studying protein dynamics is crucial for many aspects of biology, from DNA hybridization, antigen-antibody binding, and DNA-protein interaction. However, one of the challenges is that the introduction of labels alter the behavior of the studied object (for instance, the diffusion coefficient changes, etc.) </p> <p> The paper by [ <a class="litnote tooltip" href="/literature/@gaster2011"> @gaster2011 <span class="tooltiptext"> Quantification of protein interactions and solution transport using high-density GMR sensor arrays </span> </a> ] focuses on using <a class="wikilink" href="/magnetic_nanoparticles/"> magnetic nanoparticles </a> in combination with <a class="wikilink" href="/giant_magnetic_resistance/"> giant magnetic resistance </a> sensors. A functionalized surface can trap the magnetic beads and their presence can be detected in parallel, in <strong> real-time </strong> . </p> <p> The core message of the work is the development of a model to characterize the protein dynamics between labeled objects and a surface. The model is semi-analytical and seems to describe properly the detected signals. </p> <p> The biggest advantage over <a class="wikilink" href="/surface_plasmon_resonance/"> surface plasmon resonance </a> sensors is the large dynamic range (6 log or more compared to 2 log for SPR), and the concentration: $$25\textrm{ng}\,\textrm{ml}^-1$$ versus $$1\textrm{pg}\,\textrm{ml}^-1$$. </p> <p> This work opens the door to profiling the affinity of specific compounds against an entire proteome. </p> <p> There is a spinout associated with the project called <a class="wikilink" href="/flux_biosciences/"> Flux Biosciences </a> </p>