Literature/202212301234 biggest challenge of single-molecule methods is non-specific binding

First published:

Last Edited:

Number of edits:

The biggest problem of single-molecule detection methods that rely on labeling specific proteins is that there will always be a "background floor" of labels that bound to the wrong protein or to the substrate.

Non-specific binding (NSB) can be worked around by coating the substrates in such a way that there can be a washing step that limits the amount of background. However, it still poses the biggest limitation for single-molecule methods.

Pushing the detection limit down to single-molecules (see: why pushing sensitivity of assays) is very promising, but must be done carefully. The interpretation of the results must be done carefully.

This point is what makes the label-free methods such a relevant tool to characterize samples at low concentrations, focusing on the intrinsic properties of the analytes.


These are the other notes that link to this one.

Nothing links here, how did you reach this page then?


Share your thoughts on this note
Aquiles Carattino
Aquiles Carattino
This note you are reading is part of my digital garden. Follow the links to learn more, and remember that these notes evolve over time. After all, this website is not a blog.
© 2021 Aquiles Carattino
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License
Privacy Policy