Literature/202301192057 free flow electrophoresis

<ul> <li>Source: [<a class="litnote tooltip" href="/literature/@krivankova1998">@krivankova1998<span class="tooltiptext">Continuous free-flow electrophoresis</span></a>]</li> <li>Tags: <a href="/tags/Free-flow-electrophoresis">#Free-flow-electrophoresis</a></li> </ul> <p><img alt="images/Screenshot 2023-01-19 at 20.59.06.png" class="wikiimage" src="/images/screenshot 2023-01-19 at 20.59.06.png" /></p> <p>The idea behind the technique is applying an electric field perpendicular to the direction of the flow of a solution containing the analyte, and the BGE, background electrolyte. Analyte is then separated based on electrophoretic mobility at the end of the channel. </p> <p>Some <strong>concerns</strong> with the method are: - Joule heating of the sample (there's some effort put on heat dissipation in the design of the instruments). This is a concern because thermal gradients will generate sample separation in directions that may not be aligned with the electrophoretic mobility. - A membrane must be used to separate the electrodes - There are some local-zone effects with the charges - Flow-rate/resolution are linked (faster allows for lower separation) - Band broadening and dilution of the separated compounds. - Differences in conductivity between sample and background electrolyte lead to <em>electrohydrodynamic</em> distortions. - Longer residence time may increase band broadening due to diffusion (but seems to be a small effect). </p> <p>There are different implementations of free-flow electrophoresis, including:</p> <ul> <li><a class="wikilink" href="/free_flow_electrophoresis/">free flow electrophoresis</a></li> <li><a class="wikilink" href="/isotachophoresis/">Isotachophoresis</a></li> <li>Free-flow <a class="wikilink" href="/isoelectric_focusing/">isoelectric focusing</a></li> <li>Recycling zone electrophoresis</li> </ul> <p>"Free flow electrophoresis is not aimed to be an analytical method. "</p>