A robust and versatile platform for image scanning microscopy enabling super-resolution FLIM
Literature Reference
Authors:
Marco Castello, Giorgio Tortarolo, Mauro Buttafava, Takahiro Deguchi, Federica Villa, Sami Koho, Luca Pesce, Michele Oneto, Simone Pelicci, Luca Lanzanó, Paolo Bianchini, Colin J. R. Sheppard, Alberto Diaspro, Alberto Tosi, Giuseppe Vicidomini
Year: 2019
Image scanning microscopy (ISM) can improve the effective spatial resolution of confocal microscopy to its theoretical limit. However, current implementations are not robust or versatile, and are incompatible with fluorescence lifetime imaging (FLIM). We describe an implementation of ISM based on a single-photon detector array that enables super-resolution FLIM and improves multicolor, live-cell and in-depth imaging, thereby paving the way for a massive transition from confocal microscopy to ISM.
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