Physical unclonable function (PUF) device

A PUF device and a method of outputting a random sequence are disclosed. The PUF device includes: at least one processing unit and at least one PUF unit, and a first PUF unit of the at least one PUF unit includes a first MOS transistor and a second MOS transistor, two sources of the two MOS transistors are connected to a same input voltage; two gates of the two MOS transistors are floating; and two drains of the two MOS transistors are respectively connected with a first processing unit, and the first processing unit is configured to: output a first random value corresponding to the first PUF unit according to a difference between two results output by the two drains of the two MOS transistors, when the input voltage is greater than or equal to a preset voltage.

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