Hplc

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High Performance Liquid Chromatography (HPLC) is a method to separate components in a liquid:

A column is filled with particles and liquid is flowed through it. Different interactions between the particles and the components of the liquid will generate a separation in time as the liquid moves through the column.

Interestingly, the properties of the column can be tuned to select for different properties (as opposed to size exclusion chromatography, that can only select for size).

HPLC in tandem with mass spectrometry is routinely used in different contexts, particularly for diagnoses.


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Aquiles Carattino
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