First published:

Last Edited:

Number of edits:

High Performance Liquid Chromatography (HPLC) is a method to separate components in a liquid:

A column is filled with particles and liquid is flowed through it. Different interactions between the particles and the components of the liquid will generate a separation in time as the liquid moves through the column.

Interestingly, the properties of the column can be tuned to select for different properties (as opposed to size exclusion chromatography, that can only select for size).

HPLC in tandem with mass spectrometry is routinely used in different contexts, particularly for diagnoses.


Share your thoughts on this note
Aquiles Carattino
Aquiles Carattino
This note you are reading is part of my digital garden. Follow the links to learn more, and remember that these notes evolve over time. After all, this website is not a blog.
© 2021 Aquiles Carattino
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License
Privacy Policy