The power of flow cytometry
The power of flow cytometry resides, first, in the wide range of cellular parameters that can be analyzed at the same time. Secondly, on the single-cell nature of the method. Flow cytometry allows not only to detect markers in average, but as a distribution, which gives access to sub-population information.
To gain access to the relevant information, several parameters can be retrieved:
- Absorption (in the sense of forward-scatter)
- Scattering (in the sense of side-scatter)
The fact that flow cytometers are commercially, robust, and versatile, are what makes them successful in clinical and biotechnological applications.
These are the other notes that link to this one.