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<ul> <li> Source: [ <a class="litnote tooltip" href="/literature/@vieillard2007"> @vieillard2007 <span class="tooltiptext"> Application of microfluidic chip with integrated optics for electrophoretic separations of proteins </span> </a> ] </li> <li> Tags: <a href="/tags/Free-flow-electrophoresis"> #Free-flow-electrophoresis </a> <a href="/tags/ffe"> #ffe </a> <a href="/tags/separation"> #separation </a> </li> </ul> <p> In this paper they built some microfluidic channel on glass (etching) and covered them with PDMS. I am not sure why they preferred to have glass/PDMS instead of glass/glass (perhaps they didn't have a way of bonding both?) </p> <p> The glass was soda-lime, the channels had a cross-section of $20\mu m \times 80\mu m$ while the PDMS had a thickness of $1mm$ . </p> <p> What I found interesting about the paper is that they used the transparent glass to couple a microscope to do real-time monitoring of the signal. They were using fluorescence emission (on an Olympus microscope), but nothing would have prevented them from using scattering or UV-Vis (except the PDMS on top.) </p> <p> Interestingly, they perform <a class="wikilink" href="/capillary_gel_electrophoresis/"> capillary gel electrophoresis </a> and <a class="wikilink" href="/capillary_zone_electrophoresis/"> capillary zone electrophoresis </a> measurements, but not <a class="wikilink" href="/free_flow_electrophoresis/"> free flow electrophoresis </a> . I wonder why they didn't attempt it. </p> <p> <img alt="Pasted image 20240428193632.png" class="wikiimage" src="/pasted image 20240428193632.png"/> </p> <p> In their system, measurements (and separation) are done as a function of time (see: <a class="wikilink" href="/time-based_separation_methods/"> Time-based separation methods </a> ). On the other hand, it allows for a very simple detection scheme, since a point detector will do. </p> <p> The schematics of their chips comes from a different paper: [ <a class="litnote tooltip" href="/literature/@mazurczyk2006"> @mazurczyk2006 <span class="tooltiptext"> A novel concept of the integrated fluorescence detection system and its application in a lab-on-a-chip microdevice </span> </a> ] </p>