Measuring size of evs with confocal microscopy

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To measure size using fluorescence of extracellular vesicles, it is possible to measure the total intensity emitted intensity of each particle, which should be proportional to the surface area[@hartjes2020EVQuant; high-throughput quantification and characterization of extracellular vesicle (sub)populations]. However, the microscope must be carefully calibrated in order to produce reliable results.


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