Multiplex bead-based flow cytometry
The core idea of the MBFCM technique is to use fluorescent beads coated with specific antigens (or anything else of interest). Then, a second reporter molecule can be used to quantify the number of proteins, cells, vesicles, etc. are attached to each bead. This is somewhat similar to the Simoa approach, but without single-molecule resolution.
The image below ([@jani2002Multiplexed immunoassays by flow cytometry for diagnosis and surveillance of infectious diseases in resource-poor settings]) shows a schematic of the idea of separating information based on fluorescence spectra.
Coupling these essays with spectrally-resolved flow cytometers, or even with imaging flow cytometer can add new dimensions to the study of small objects (such as extracellular vesicles).
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