Tr-fia

First published:

Last Edited:

Number of edits:

Time-resolved fluorescence immunoassay: TR-FIA.

It is similar to Elisa test, but using a fluorescent label such as europium. I am not sure what the "Time-resolved" part of the essay entails.

Check: Wallac Victor 2 (device on which some tests are performed[@duijvesz2015aImmuno-based detection of extracellular vesicles in urine as diagnostic marker for prostate cancer: Extracellular vesicles as marker for PCa]) ( #todo )

One of the challenges of the method is that the signal's strength also depends on the number of antigens per vesicle and the affinity of the antibody to the epitope[@duijvesz2015aImmuno-based detection of extracellular vesicles in urine as diagnostic marker for prostate cancer: Extracellular vesicles as marker for PCa].

Another challenge is that some viruses also contain CD9 and CD63, the markers normally used for the detection of extracellular vesicles (see: HIV-1 contains CD9 and CD63)


Comment

Share your thoughts on this note
Aquiles Carattino
Aquiles Carattino
This note you are reading is part of my digital garden. Follow the links to learn more, and remember that these notes evolve over time. After all, this website is not a blog.
© 2021 Aquiles Carattino
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License
Privacy Policy